Fate of Transforming Deoxyribonucleic Acid After Uptake by Competent Bacillus subtilis : Nonrequirement of Deoxyribonucleic Acid Replication for Uptake and Integration of Transforming Deoxyribonucleic Acid
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منابع مشابه
Fate of Transforming Deoxyribonucleic Acid in Bacillus Subtilis.
Venema, G. (Hammersmith Hospital, London, England), R. H. Prtichard, and T. Venema-Schröder. Fate of transforming deoxyribonucleic acid in Bacillus subtilis. J. Bacteriol. 89:1250-1255. 1965.-Donor deoxyribonucleic acid (DNA) introduced into competent recipient cells of Bacillus subtilis undergoes a transient loss of its transforming activity shortly after uptake; transforming activity reappear...
متن کاملProperties of Newly Introduced Transforming Deoxyribonucleic Acid in Bacillus Subtilis.
Venema, G. (Hammersmith Hospital, London, England), R. H. Pritchard, and T. Venema-Schröder. Properties of newly introduced transforming deoxyribonucleic acid in Bacillus subtilis. J. Bacteriol. 90:343-346. 1965.-In these experiments, deoxyribonucleic acid (DNA) with donor markers, re-extracted shortly after uptake by competent Bacillus subtilis cells, had abnormal physical properties as compar...
متن کاملSingle-stranded regions in transforming deoxyribonucleic acid after uptake by competent Haemophilus influenzae.
About 15% of donor deoxyribonucleic acid (DNA) is single stranded immediately after uptake into competent Haemophilus influenzae wild-type cells, as judged by its sensitivity to S1 endonuclease. This amount decreases to 4 to 5% by 30 min after uptake. Mutants which are defective in the covalent association of recipient and donor DNA form little or no S1 endonuclease-sensitive donor. At 17 C don...
متن کاملDeoxyribonucleic acid-deoxyribonucleic acid hybridization assay for replication origin deoxyribonucleic acid of Escherichia coli.
Deoxyribonucleic acid (DNA)-DNA hybridization on nitrocellulose filters can be used to assay for replication origin DNA from Escherichia coli if the DNA attached to the filters is enriched for the replication origin sequences. Such DNA can be readily isolated from very rapidly growing cells. When low amounts of this DNA were attached to filters, radioactively labeled DNA from the replication or...
متن کاملMechanism of inactivation of transforming deoxyribonucleic acid by X rays.
Transforming deoxyribonucleic acid (DNA) from Haemophilus influenzae was exposed to X rays either in phosphate buffer or in 10% yeast extract. Relations between determinations of biological inactivation, DNA uptake by competent H. influenzae, integration of DNA into the competent cell genome, and induced single-and double-strand breaks indicate that transforming DNA is inactivated by the direct...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1973
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.113.3.1512-1514.1973